The 4½ day training course stresses public involvement, safety, and application restrictions and techniques from the new 2nd edition AFS Rotenone SOP Manual and is taught by manual’s two senior authors. The course was developed to meet the U.S. Environmental Protection Agency’s labeling requirements that include the AFS Rotenone SOP Manual. The course uses classroom lectures, laboratory and field exercises, participant presentations, classroom discussions, and quizzes on the content as instructional methods. Topics include: soliciting public involvement; fisheries management/conservation plans; species sensitivities to chemicals; selective versus eradication treatments; detecting and treating upwelling groundwater; use of GPS technology in planning treatments; proper safety equipment; rotenone and potassium permanganate chemistry, toxicology and environmental behavior; reading and following labels and SDSs; instructive project case histories; crisis management strategies; and characteristics of successful projects. The participants perform laboratory, field and planning exercises during the course including performing toxicity tests on rotenone and potassium permanganate at realistic concentrations, performing calculations to determine amount of chemicals needed to treat flowing and standing waters, constructing and operating application and deactivation equipment, and developing public involvement, application, deactivation, safety and monitoring plans. Participants receive a copy of the AFS Rotenone SOP Manual published in 2018, and successful completion of a final exam will give the participant a certificate of completion from AFS.