Use of Genetic Microsatellite Markers to Identify Factors Affecting Stocking Success in Striped Bass
Tanya L. Darden, Forrest Sessions, and Michael R. Denson
Abstract.—The emerging use of genetic markers for stock identification is advantageous as this technique utilizes an inherent permanent mark, it eliminates stress associated with conventional tagging methods, and mark recovery is nonlethal. In controlled experiments, increased accuracy and reliability have been demonstrated with genetic tags, as compared to other commonly used markers. While a variety of genetic markers exist for fish identification, microsatellites are preferred because of their polymorphic nature and versatile use, including identification of population structure, measures of genetic diversity, and parentage analysis. Microsatellites provide a cost-effective tool for a variety of applications, including their use in complex experimental designs, as well as responsible genetic population management. We provide examples of the use of microsatellites as genetic tags in South Carolina striped bass Morone saxatilis, describing an experimental restoration effort in the Ashley River and a large-scale stock enhancement effort for the Santee-Cooper reservoirs.