Effect of Dietary Amprolium on Egg and Tissue Thiamine Concentrations in Lake Trout
D. C. Honeyfield, K. Fynn-Aikins, J. D. Fitzsimons, and J. A. Mota
Abstract.—Dietary amprolium, a thiamine antagonist, was fed to lake trout Salvelinus namaycush broodstock from April to October before spawning to determine its effect on egg and tissue concentrations of thiamine, thiamine monophosphate, and thiamine pyrophosphate. The thiamine concentration of eggs from fish fed no amprolium was 61.8 nmol/g, whereas the concentration of thiamine in fish fed 0.05 and 0.10% amprolium was 4.02 and 1.71 nmol/g (P < 0.01), respectively. In lake trout fed 0.10% amprolium beginning in August, egg free thiamine concentration was reduced to 11.6 nmol/g. No sign of early mortality syndrome was observed in sac fry from eggs in this study, which suggests that thiamine concentrations in the egg were not low enough to be below a critical threshold or that factors other than thiamine are involved in early mortality syndrome.