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bluebook

Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens, Blue Book 2012 Edition (3-edition subscription)

$150.00

SKU: 70314S. Category: .

Product Description

CD-ROM

Published by the American Fisheries Society

Publication date: January 2013

ISBN: 978-1-934874-33-2

Summary

Originally published in loose-leaf format as the Blue Book, this updated, searchable CD contains both the familiar chapters of the Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens and the newly revised Standard Procedures for Aquatic Animal Health Inspections coauthored by the U.S. Fish and Wildlife Service and the AFS Fish Health Section. This new format allows the user to easily access information and contains numerous color photographs and video clips.

CONTENTS

Section 1

Diagnostic Procedures for Finfish and Shellfish Pathogens

Chapter 1 Bacterial Diseases of Fish

1.1 GENERAL PROCEDURES

1.1.1 General Procedures for Bacteriology

1.1.2 Flow Chart for the Presumptive Identification of Selected Bacteria from Fishes

1.2 GRAM-NEGATIVE BACTERIAL DISEASES

1.2.1 Bacterial Gill Disease

1.2.2 Coldwater Disease

1.2.3 Columnaris Disease

1.2.4 Edwardsiella tarda Septicemia

1.2.5 Enteric Redmouth Disease

1.2.6 Enteric Septicemia

1.2.7 Furunculosis

1.2.8 Other Diseases Caused by Aeromonas salmonicida

1.2.9 Motile Aeromonas Septicemia

1.2.10 Vibriosis

1.2.11 Coldwater Vibriosis

1.2.12 Francisellosis

1.2.12.1 Worksheet A

1.2.12.2 Worksheet B

1.2.13 Piscirickettsiosis

1.2.14 Photobacteriosis

1.3 GRAM-POSITIVE BACTERIAL DISEASES

1.3.1 Bacterial Kidney Disease

1.3.1.1 BKD Methods Appendix 1

1.3.1.2 BKD Methods Appendix 2

1.3.2 Pseudokidney Disease

1.3.3 Streptococcal Disease

Chapter 2 Viral Diseases of Fish

2.1 GENERAL PROCEDURES FOR VIROLOGY

2.2 SPECIFIC VIRAL DISEASES

2.2.1 Channel Catfish Virus Disease

2.2.2 Erythrocytic Inclusion Body Syndrome

2.2.3 Herpesvirus Diseases of Salmonids

2.2.4 Infectious Hematopoietic Necrosis

2.2.5 Infectious Pancreatic Necrosis

2.2.6 Viral Erythrocytic Necrosis

2.2.7 Viral Hemorrhagic Septicemia

2.2.8 Infectious Salmon Anemia

2.2.8.1 ISA Appendix 1

2.2.8.2 ISA Appendix 2

2.2.9 Spring Viremia of Carp

2.2.10 Koi Herpesvirus Disease

2.2.11 Goldfish Herpesviral Hematopoietic Necrosis

2.2.12 Largemouth Bass Virus Disease

2.2.13 Oncorhynchus masou virus Disease

2.3 OTHER VIRAL DISEASES

2.3.1 Other Viruses Isolated from Fish

2.A1 METHODS FOR TESTING FOR THE PRESENCE OF MYCOPLASMA IN STOCK CELL CULTURES APPENDIX 1

Chapter 3 Parasitic Diseases of Fish

3.1 GENERAL PROCEDURES

3.1.1 General Procedures for Parasitology

3.1.2 Key to Major Taxa of Adult Parasites of Fishes

3.2 SPECIFIC PARASITIC DISEASES

3.2.1 Ichthyobodiasis

3.2.2 Diplomonad (Hexamitid) Flagellates: Diplomonadiasis, Hexamitiasis, Spironucleosis

3.2.3 Ovipleistophoriasis: A Microsporidian Disease of the Golden Shiner Ovary

3.2.4 Salmonid Ceratomyxosis

3.2.4.1 Salmonid Ceratomyxosis Methods Appendix 1

3.2.5 Whirling Disease of Salmonids

3.2.6 Proliferative Gill Disease

3.2.6.1 Proliferative Gill Disease Methods Appendix 1

3.2.7 Proliferative Kidney Disease

3.2.8 Ichthyophthiriasis

3.2.9 External Infection by Ciliated Parasites

3.2.10 Monogenean Diseases

3.2.11 Bothriocephalosis

3.2.12 Lernaeid Parasitism

3.2.13 Gill Maggot Disease (Genus Salmincola)

3.2.14 Sea Lice Parasitism

3.2.15 Branchiuran Fish Louse Disease

3.2.16 Centrocestiasis

3.2.17 Salmonid Intranuclear Microsporosis

3.2.17.1 Nested PCR: Nucleospora (Enterocytozoon) salmonis

3.2.17.2 Quantitative PCR: Nucleospora (Enterocytozoon) salmonis

3.2.18 Ichthyophonus Disease

Chapter 4 Mycotic Diseases of Fish

4.1 GENERAL PROCEDURES FOR MYCOLOGY

4.2 SPECIFIC DISEASES

4.2.1 Branchiomycosis

4.2.2 Saprolegniasis

4.3 MISCELLANEOUS MYCOTIC DISEASES

Chapter 5 Diseases of Molluscan Shellfish

5.1 DISEASES OF SHELLFISH INTRODUCTION

5.2 SPECIFIC DISEASES

5.2.1 Perkinsus marinus Infection of American Oysters

5.2.2 Haplosporidiosis of American Oysters

5.2.3 Oyster Seaside Haplosporidiosis

5.2.4 Velar Virus Disease of Pacific Oysters

5.2.5 Mikrocytosis (Denman Island Disease)

5.2.6 Nocardiosis of Pacific Oysters

5.2.7 Bonamiasis of Ostreid Oysters

5.2.8 Hexamitiasis of Oysters

5.2.9 Marteiliasis of Ostrea edulis

5.2.10 Shell Disease of Oysters

5.2.11 Disseminated Neoplasia of Bivalve Molluscs

5.2.12 Miscellaneous Diseases of Molluscs

5.2.13 Juvenille Oyster Disease

Chapter 6 Diseases of Crustaceans

6.2 SPECIFIC DISEASES

6.2.1 Necrotizing Hepatopancreatitis Disease of Peneaid Shrimp

6.2.2 Taura Syndrome of Peneaid Shrimp

6.2.3 Lagenidiasis of Decapod Crustaceans

6.2.4 White Spot Syndrome

Section 2
USFWS/AFS-FHS Standard Procedures for Aquatic Animal

Health Inspections

Chapter 1 Introduction

1.1 Introduction

Chapter 2 Sampling

2.1 Sampling Introduction

2.2 Sampling

2.3 Reagents, Media, and Media Preparation

2.4 Glossary

2.5 References

Chapter 3 Bacteriology

3.1 Bacteriology Introduction

3.2 Aeromonas salmonicida (Furunculosis)

3.3 Yersinia ruckeri (Enteric Redmouth Disease, ERM)

3.4 Edwardsiella ictaluri (Enteric Septicemia of Catfish, ESC)

3.5 Renibacterium salmoninarum (Bacterial Kidney Disease, BKD)

3.6 Piscirickettsia salmonis

3.7 Reagents, Media, and Media Preparation

3.8 Bacterial Identification Techniques

3.9 Glossary

3.10 References

3.A1 Laboratory Reference Flow Chart Appendix 1

3.A2 Profiles Obtained with API-20E for Known Fish Pathogens

3.A3.A Worksheet A – PCR Sample Data/Log Sheet

3.A3.B Worksheet B – Initial Amplification of Nucleic Acid by PCR for the
Confirmation of R. salmoninarum

3.A3.C Worksheet C – Nested Amplification of Nucleic Acid by PCR for the
Confirmation of R. salmoninarum

3.A3.D Worksheet D – Initial Amplification of Nucleic Acid by PCR for the
Confirmation of P. salmonis

3.A3.E Worksheet E – Nested Amplification of Nucleic Acid by PCR for the
Confirmation of P. salmonis

3.A3.F Worksheet F – Direct Amplification of Nucleic Acid by PCR for the
Confirmation of P. salmonis

3.A3.G Worksheet G – Photodocumentation of the PCR Product Gel

Chapter 4 Virology

4.1 Virology Introduction

4.2 Selection of Appropriate Cell Lines

4.3 Cell Culture

4.4 Sample Processing Procedures

4.5 Screening Method for Viral Isolation

4.6 Identification of Viruses

4.7 Serum Neutralization

4.8 Indirect Fluorescent Antibody (IFAT) Procedure

4.9 Reagents and Media

4.10 Glossary

4.11 References

4.A1.A Worksheet A – PCR Sample Data/Log Sheet

4.A1.B Worksheet B – Amplification of Nucleic Acid by PCR for the Confirmation of
Viral Fish Pathogens

4.A1.B.1 Worksheet B.1 – Infectious Hematopoietic Necrosis Virus (IHNV)

4.A1.B.2 Worksheet B.2 – Infectious Pancreatic Necrosis Virus (IPNV)

4.A1.B.3 Worksheet B.3 – Infectious Salmon Anemia Virus (ISAV)

4.A1.B.4 Worksheet B.4 – Largemouth Bass Virus (LMBV)

4.A1.B.5 Worksheet B.5 – Oncorhynchus masou Virus (OMV)

4.A1.B.6 Worksheet B.6 – Spring Viremia of Carp Virus (SVCV)

4.A1.B.7 Worksheet B.7 – Viral Hemorrhagic Septicemia Virus (VHSV)

4.A1.C Worksheet C – Photodocumentation of the PCR Product Gel

4.A2 Cell Enumeration

Chapter 5 Parasitology

5.1 Parasitology Introduction

5.2 Myxobolus cerebralis (Whirling Disease)

5.3 Ceratomyxa shasta (Ceratomyxosis)

5.4 Tetracapsula bryosalmonae (Proliferative Kidney Disease)

5.5 Bothriocephalus acheilognathi (Bothriocephalosis, Asian Tapeworm)

5.6 Media and Reagents

5.7 Glossary

5.8 References

Chapter 6 Polymerase Chain Reaction (PCR)

6.1 Polymerase Chain Reaction (PCR) Introduction

6.2 PCR – Quality Assurance/Quality Control

6.3 PCR Protocols

6.4 Reagents

6.5 Analysis of Extracted DNA Using an UV Spectrophotometer

6.6 Table of PCR Protocols

6.7 References

Appendix 1 The Handbook and Oversight Committee

A1.1 Format of the Handbook

A1.2 Handbook Revision and Oversight Committee

A1.3 Process for Amending the Handbook

Appendix 2 Handbook Committee Members

A2.1 2000 – 2002 Handbook Development Committee A2.2 2002 – 2003 Handbook Development Committee A2.3 2003 – 2004 Handbook Development Committee A2.4 2004 – 2005 Handbook Development Committee A2.5 2006 – 2007 Handbook Development Committee A2.6 2008 – 2010 Handbook Development Committee

A2.7 2011 – 2012 Handbook Development Committee

Appendix 3 Position Statements

A3.1 Position Statements Introduction A3.2 Sampling Position Statements A3.3 Bacteriology Position Statements A3.4 Virology Position Statements
A3.5 Parasitology Position Statements

A3.6 Polymerase Chain Reaction (PCR) Position Statements (none)

A3.7 Appendix 1 The Handbook and Oversight Committee Position Statements

Section 3

Quality Assurance/Quality Control

C.1 Quality Assurance/Quality Control